Goldner,+Nick

9/6 we used .5% agarose gel and a high KB ladder to try and resolve the genomic DNA. however our gel did not resolve

9/8 Today Miranda and I have found and isolated 3 CNS's and their primers. We are creating a joint effort on CNS 1 while Miranda is taking CNS 2 and I, CNS3.
 * 1) CNS 1-3 DNA
 * 2) [[file:Gene CNS1-3.pdf]]
 * 3) CNS 1
 * 4) [[file:CNS1 DNA seq.docx]]
 * 5) [[file:CNS1 Primer.docx]]
 * 6) [[file:CNS1.pdf]]
 * 7) CNS 2
 * 8) [[file:CNS2 DNA seq.docx]]
 * 9) [[file:CNS2 Primer.docx]]
 * 10) [[file:CNS2.pdf]]
 * 11) CNS3
 * 12) [[file:CNS3 DNA seq.docx]]
 * 13) [[file:CNS3 Primer.docx]]
 * 14) [[file:CNS3.pdf]]

9/19 today I ran the pcr for CNS1 and CNS3 row one is orange row two is blue

A 60.5 B 60.2 C 59.6 D 58.6

9/28

ran pcr

a 60.1 b 59.9 c 59.4 d 58.7

Transformation unsucesffuly.

Sunday, December 18, 2011 Revise protocol using the same primers.

1.